The basic objective is to measure the cholesterol level in blood. An approach towards estimation of cholesterol was done by setting up a simple redox reaction. The cholesterol oxidase enzyme 8 breaks down the substrate into an intermediate cholest-5-en-3-one, which is then converted to cholest-4-en-3-one 15. In the present study this mechanism was exploited. The current produced by the release of electrons from the above reaction was determined by the Op-Amp and the pre-programmed micro-controller was used to determine the cholesterol concentration. And the cholesterol concentration was displayed in 16x2 alphanumeric display.
The Op-Amp is used to amplify the signal which is fed to microcontroller, a predefined calculation was embedded in the controller with programming and the result is displayed in the LCD.
Measurement of serum cholesterol levels can serve as an indicator of liver function, biliary function, intestinal absorption, propensity toward coronary artery disease, and thyroid function. Cholesterol levels are important in the diagnosis and classification of hyperlipoproteinemias. Stress, age, gender, hormonal balance, and pregnancy affect normal cholesterol levels. The Adult Treatment Panel of the National Cholesterol Education Program (NCEP) recommends that all adults 20 years of age and over should have a fasting lipoprotein profile (total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride.